- Exit-of-mitosis Mutants
- cdc14-ts
- cdc14-ts GAL-SIC1
- cdc14ts then GAL-SIC1
- cdc14-ts cdh1∆
- cdc14-ts sic1∆
- cdc14-ts GAL-CLN2
- net1-ts
- net1-ts in nocodazole
- net1∆ cdc20-ts
- tem1∆ net1-ts
- cdc15∆ net1-ts
- cdc15∆ net1-ts cdh1∆
- GAL-NET1
- GAL-CDC14
- GAL-NET1 GAL-CDC14
- cdc15-ts multicopy CDC14
- tem1-ts multicopy CDC14
- TAB6-1
- TAB6-1 cdc15∆
- TAB6-1 clb5∆ clb6∆
- TAB6-1 CLB1 clb2∆
net1-ts
debug: ,
test user =
test db =
Simulation:
Change of parameters: kasrent=1, kasrentp=0.005 (=0.005X WT)
Length of G1 phase: 42.1 min.
Mass at division: 6.30.
Experiments:
Shou, W., Seol, J.H., Shevchenko, A., Baskerville, C., Moazed, D., Chen, Z.W.S., Jang, J., Shevchenko, A., Charbonneau, H. and Deshaies, R.J. (1999). Exit from mitosis is triggered by Tem1-dependent release of the protein phosphatase Cdc14 from nucleolar RENT complex. Cell 97:233-244.
[Abstract] [Article]
[Abstract] [Article]
Visintin, R., Hwang, E.S., Amon, A. (1999). Cfi1 prevents premature exit from mitosis by anchoring Cdc14 phosphatase in the nucleolus. Nature 398:818-823.
[Abstract] [Article]
[Abstract] [Article]
Experimental results: Visintin: Fig. 4, viable, but growth is retarded. It shows a 15 min delay in bud formation, 30 min delay in DNA replication and entry into mitosis.
Comments:
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